EO-3 oil from Kentucky Equine Research, is a potent, effective source of long-chain omega-3 fatty acids. Analysis has shown that EO-3 has the highest percentage of long-chain omega-3 fatty acids as well as the highest ratio of omega-3 to omega-6 fatty acids when compared to other commonly fed oils.

Feeding Directions

Add 1 to 2 oz (30 to 60 mL) to the feed. For best results, introduce EO-3 to the ration gradually over a period of 5 to 10 days.

In racehorses, we recommend stopping this product 24 hours prior to racing. Why?

Guaranteed Analysis	Concentration	Per 30 mL
Fat	98%	29.4 g
Omega-3 fatty acids	35%	9,450 mg
DHA and EPA	25%	6,750 mg
Omega-6 fatty acids	3%	810 mg

Why Choose EO-3?

Palatable highly refined and flavored marine-derived oil

Improves glucose tolerance

Strengthens immune function

Increases red blood cell flexibility

Enhances bone metabolism and development

Benefits of DHA- and EPA-mediated inflammatory response include reduction in joint inflammation, allergic reactivity, exercise-induced bronchoconstriction, and exercise-induced pulmonary hemorrhage

Reproductive benefits include improved fertility, improved colostrum quality, enhanced passive transfer of antibodies to foals, and increased sperm concentration, motility, and viability

Marine-derived long-chain fatty acids DHA and EPA are more efficiently used as precursors of local hormones than plant-based sources of omega-3 fatty acids

ACVIM consensus statement recommends DHA and EPA supplementation for horses with inflammatory airway disease/equine asthma

Research

Researchers have focused their attention on two distinct families of longchain polyunsaturated fatty acids (PUFA): the omega-3 family and the omega-6 family. The omega-3 family stems from alpha-linolenic acid (ALA), and the omega-6 family originates from linoleic acid (LA). The term omega-3 is in reference to the location of the first double bond positioned three carbon atoms from the terminal end of the fatty acid chain. Marine-derived oils are rich in the longer chain omega-3 fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), 20 and 22 carbon atoms, respectively, while ALA, containing 18 carbon atoms, can be found in plant sources such as flax, soybean, and canola. ALA and LA are considered “essential fatty acids” because they are instrumental in the life cycle, yet they cannot be manufactured in the body and must be obtained from dietary sources.

Omega-3 Fatty Acids

Significant members of the omega-3 family are EPA, DHA, and ALA. EPA and DHA are found almost exclusively in fish, namely cold-water species, since they are at the top of a food chain based largely on algae that manufacture EPA and DHA. On the other hand, ALA is found predominantly in leafy plants and flaxseed (linseed).

Omega-6 Fatty Acids

The primary source of omega-6 fatty acids in the diet is LA derived from the oils of seeds and grains. Corn, sunflower, and safflower oil contain abundant quantities of LA. Arachidonic acid (AA) is an intermediate in the metabolism of LA to the various cytokines termed “pro-inflammatory.”

The Omega-3 to Omega-6 Ratio: A Balancing Act

Omega-3 and omega-6 fatty acids must be balanced within the body in order for both to be effective. The significant biological difference between omega- 3 fatty acids and omega-6 fatty acids is related to their roles as precursors of inflammatory and immune intermediaries. For example, each fatty acid is necessary for the production and distribution of prostaglandins. The prostaglandins that evolve from consumption of omega-3 and omega-6 fatty acids have different effects on inflammation processes in the body.

The omega-3 fatty acids, EPA and DHA, are precursors of a class of inflammatory compounds that yield a milder response than those produced from omega- 6 fatty acids. In other words, the inflammatory response from cytokines and prostaglandins that originate from these omega-3 fatty acids is moderate compared to the response from cytokines and prostaglandins derived from omega-6, which are considered “pro-inflammatory” by comparison.

Scientists have not pinpointed the optimal ratio of omega-3 fatty acids to omega-6 fatty acids for horses of various ages and uses, though they believe a ratio of 2 to 4:1 may be optimal. Even without an exact ratio, general knowledge of omega-3 and omega-6 fatty acids and typical equine management practices uncover some undesirable trends.

Feedstuffs have varying levels of omega-3 and omega-6 fatty acids. The natural diet of horses—primarily fresh and dried forages—contains more omega- 3 fatty acids than diets consisting of a mixture of forage and cereal grains. Domesticated horses are frequently given concentrated sources of energy in the form of grain meals. Grains possess more omega-6 fatty acids than forage. Performance horses are often fed supplemental sources of fat to add much needed calories to their rations. Corn oil, probably the most popular fat supplement offered to horses, as well as safflower oil, canola oil, and sunflower oil, is rich in omega-6 fatty acids. Feeding one or more of these, especially in combination with a high-grain diet, may supply a horse with a surplus of omega-6 fatty acids, skewing the ratio of omega-3 to omega-6 fatty acids and creating a balance of omega-3 to omega-6 fatty acids that may be inappropriate. Such diets may not be as beneficial as one abundant in omega-3 fatty acids.

EO•3 – Preferential Source for Omega-3 Fatty Acid Supplementation

Plant sources of omega-3 fatty acids such as flaxseed (linseed) are inferior sources due to inherent metabolic disadvantages. Even though EPA and DHA (20 and 22 carbon, respectively) can be formed in vivo by elongation of alpha-linolenic acid (ALA) (18 carbon), this process is inefficient and complicated. In addition, the enzymes used in the metabolism of ALA to EPA and DHA are the same enzymes used to elongate LA to AA. When horses consume feeds with greater quantities of LA (omega-6) than ALA (omega-3), the concomitant conversion of LA (18 carbon-omega-6) to AA (20 carbon–omega-6) results in the production of more pro-inflammatory cytokines and prostaglandins.

Only fish oils are direct sources of EPA and DHA. In the past, these oils were typically not as palatable as those that provide more generous quantities of omega-6 fatty acids. However, recent studies at Kentucky Equine Research (KER) have shown that advances in processing technology have overcome the palatability issue inherent in the use of fish oil.

Polyunsaturated Fatty Acid Composition of Commonly Used Oils

Oil Type	Omega-3 Concentration	Omega-3:Omega-6	Predominant Omega-3 Source
EO•3	35%	12	EPA and DHA
Flax oil	54%	4.15	ALA
Soy oil	7%	0.14	ALA
Rice bran oil	1%	0.03	ALA
Corn oil	1%	0.02	ALA

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